Journal: Molecular Medicine Reports

Article Title: Effects of tripterygium glycosides on restenosis following endovascular treatment

doi: 10.3892/mmr.2016.5149

Figure Lengend Snippet: Post-operative protein expression of PCNA, VEGF, CDK2 and p21 Cip1 (optical density) in 24 rabbits.

Article Snippet: The sections were subsequently incubated with primary antibody at 4°C overnight, and then with secondary antibody at room temperature for 15 min. Primary antibodies included: 0.1 ml (100 μ g/ml) of monoclonal mouse anti-PCNA (1:5,000; Wuhan Boster Biological Technology, Inc., Wuhan, China; cat. no. 610664), 0.1 ml (100 μ g/ml) of polyclonal rabbit anti-VEGF (1:200; Abcam, Cambridge, MA, USA; cat. no. ab46160), 0.1 ml (100 μ g/ml) of polyclonal rabbit anti-cyclin-dependent kinase 2 (CDK2; 1:200; Wuhan Boster Biological Technology, Inc.; cat. no. 8233343), and 0.1 ml (100 μ g/ml) of monoclonal mouse anti-cell-cycle regulatory protein (p21 Cip1 ; 1:250; Wuhan Boster Biological Technology, Inc.; cat. no. 554262).

Techniques: Expressing, Control